Table 2 Descriptive and result table of 26 studies categorized as proof-of-concept research
First author Year | Sample types Microsample details: - Volume of blood - Punch diameter or area (DBS only) - Collection device (manufacturer) | Microsample collection method | Notable sample preparation | Acquisition method | Effective gradient length (min) | Throughput Quantification | |
|---|---|---|---|---|---|---|---|
Targeted | Untargeted | ||||||
Chambers [28] 2013a | DBS/DPS/DSS - 15 µL - Entire spot - 903 Protein Saver (Whatman) Plasma Serum WB | Venous blood deposited onto DBS | NA | NA | DDA Orbi-q-IT | 90 | 253 proteins (1549 peptides) No quantification |
Chambers [50] 2013b | DBS - 15 µL - Entire spot - 903 Protein Saver (Whatman) | Venous blood deposited onto DBS | NA | MRM | NA | NA | 37 proteins (40 peptides) Calibration curve with SIS peptides |
Martin [29] 2013 | DBS - Ahlstrom grade 226 filter paper (ID Biological Systems) | Finger prick | Liquid extraction surface analysis to extract proteins | NA | DDA Orbi-q-IT | 30 | 120 proteins No quantification |
Chambers [49] 2015 | DBS - 15 µL - Entire spot - 903 Protein Saver (Whatman) | Venous blood deposited onto DBS | NA | MRM | NA | NA | 97 proteins (169 peptides) PAR relative to SIS peptides LLOQ: 190 ng/mL (cholinesterase) |
Geyer [39] 2016 | Plasma - 1 µL - Custom pipette tip-based centrifugal devices | Finger prick | Basic reverse-phase fractionation (high throughput method) | NA | DDA Orbi-q-Orbi | 15 100 | 347 proteins (short gradient) 1040 proteins (long gradient) LFQ |
Cox [46] 2017a | DBS - 20 µL - Entire spot - DMPK-C (Whatman) | Venous blood deposited onto DBS | Buffer washing to enrich membrane proteins | PRM | NA | NA | 4 proteins (4 peptides) PAR relative to SIS peptides |
Henderson [44] 2017 | DBS - 65 µL - 1 × 3.2 mm - 903 Protein Saver (Whatman) Plasma | Finger prick by trained personnel Venous blood deposited onto DBS | NA | PRM | NA | NA | 3 proteins (6 peptides) Peak area integration of transitions LLOQ: 8.3 mg/dL (apolipoprotein B) |
Ozcan [53] 2017 | DBS - 1 × 3 mm - 903 Protein Saver (Whatman) Serum | Finger prick | NA | MRM | NA | NA | 82 proteins (156 proteins) PAR relative to SIS peptides |
van den Broek [88] 2017 | Plasma VAMS - 10 µL - Mitra (Neoteryx) | VAMS dipped into venous blood | NA | SRM | DIA SWATH-MS | 60 | SRM: 6 proteins (10 peptides) PAR relative to SIS peptides DIA: 423 proteins (1661 peptides) LFQ |
Wouters [89] 2017 | DBS - 10 µL - Entire spot - Human ID Blood stain Card BFC 180 (Whatman) | Finger prick | Immobilized enzyme reactor for digestion | NA | DDA TOF-q-TOF | 45 | 156 proteins No quantification |
Forchelet [37] 2018 | Microsample - 20–25 µL resulting in 2 µL sample - Custom microfluidic device Plasma Serum | Finger prick | NA | NA | DIA SWATH-MS | ND | 312 proteins LFQ |
Rosting [90] 2018 | DBS - Entire spot - Ahlstrom grade 226 (ID Biological Systems) | Finger prick | Liquid surface extraction analysis to extract proteins | NA | DDA Orbi-q-IT | 30 | 350 proteins (1065 peptides) No quantification |
Samenuk [91] 2019 | NBS - ¼ of spot | ND | NA | NA | MALDI-TOF | ND | 30 peptides No quantification |
Vidova [52] 2019 | DBS - 1 × 3 mm - 903 Protein Saver (Whatman) Serum WB | Finger prick | NA | SRM | NA | NA | 7 proteins (13 peptides) PAR relative to SIS peptides LLOQ: 0.8 nM (serum amyloid A4) |
Eshgi [41] 2020 | DBS - 50 µL - 5 × 6 mm - 903 Protein Saver (Whatman) WB | Venous and capillary blood deposited onto DBS | NA | MRM | NA | NA | 245 proteins PAR relative to SIS peptides LLOQ: 0.2 fmol |
Nakajima [42] 2020 | DBS - 2 × 3.2 mm - Blood sampling paper (Advantec) | Finger prick | Sodium carbonate precipitation to reduce soluble proteins | NA | DIA Orbi-q-Orbi | 75 | 1977 proteins LFQ |
van den Broek [92] 2020 | VAMS - 10 µL - Mitra (Neoteryx) | Finger prick, both self-sampled and by trained personnel | NA | SRM | NA | NA | 11 proteins (22 peptides) PAR relative to SIS peptides |
Cox [47] 2021 | DBS - 20 µL - Entire spot - DMPK-C (Whatman) | Venous and capillary blood deposited onto DBS | Buffer washing to enrich membrane proteins | PRM | NA | NA | 4 proteins (4 peptides) PAR relative to SIS peptides LLOQ: 0.135 nM (CD71) |
Mc Ardle [93] 2022 | Plasma VAMS - 10 µL - Mitra (Neoteryx) | VAMS dipped into venous blood | NA | NA | DIA Orbi-q-Orbi | 21 60 | 939 proteins LFQ |
Schneider [94] 2022 | DBS - 50 µL - 1 × 3 mm - 903 Protein Saver (Whatman) | Venous blood deposited onto DBS | NA | NA | DIA Orbi-q-Orbi | 90 |  ~ 400 proteins (~ 3000 peptides) LFQ, peptide ratios |
Whelan [30] 2023 | Plasma VAMS - 10 µL - Mitra (Neoteryx) | Finger prick, self-sampled VAMS dipped into venous blood | NA | MRM | DIA Orbi-q-Orbi | 60 | MRM: 60 proteins (114 peptides) PAR relative to SIS peptides DIA: 4661 proteins No quantification |
Brockbals [35] 2024 | DBS - 20 µL - 1 × 3 mm - 903 Protein Saver (Whatman) | Venous and capillary blood deposited onto DBS | NA | MSMS with inclusion list | NA | NA | 3 proteins (11 peptides) Peptide ratios |
Richard [95] 2024 | DBS - 20 µL - 4 × 6 mm - 903 Protein Saver (Whatman) | Finger prick | NA | MRM | NA | NA | 250 proteins (319 peptides) PAR relative to SIS peptides |
Shen [96] 2024 | VAMS - 10 µL - Mitra (Neoteryx) | Finger prick, self-sampled | Biphasic MTBE extraction to precipitate protein pellet | NA | DIA SWATH-MS | 43 | 291 proteins LFQ |
Sun [51] 2024 | Blood cells DBS - 100 µL - Entire spot - FTA card (Qiagen) Plasma WB | Venous blood deposited onto DBS | NA | NA | DDA Orbi-q-Orbi | 78 | 758 proteins (7348 peptides) LFQ |
Vegesna [97] 2025 | DBS - 903 Protein Saver (Whatman) VAMS - Mitra (Neoteryx) Plasma | VAMS dipped into whole blood or plasma Venous blood deposited onto DBS | NA | NA | DIA Orbi-q-Orbi | 60 | ND LFQ |