Fig. 2
From: Multiplex quantification of endocrine proteins in volumetric dried blood spots
Comparison of protein levels in EDTA plasma and qDBS. Five proteins were measured in one multiplexed immunoassay (Luminex) to compare the concentrations between eluates from qDBS and EDTA plasma samples: a LHB; b FSHB; c PRL; d TSHB; e GH1. Left column: Dose–response curve of plate 1 with a subset of subjects (n = 33); qDBS samples are depicted in yellow, plasma in light blue, and values from the calibration curve in black. The limit of quantification (LOQ) is shown by a black dashed line. Center column: protein concentrations in paired qDBS and plasma samples are adjusted for the preparatory dilution factors. Right column: The correlation between plasma and qDBS for all subjects (n = 100) above LOQ and below the highest point on the calibration curve. The blue line shows the linear regression model, and the 95% confidence intervals are in grey. Pearson r demonstrates the correlation, and the identity line is shown in black