Fig. 1

Study design—a Venous blood samples were collected from 100 participants in EDTA tubes as part of clinical routine procedures. b Whole blood was transferred onto qDBS cards and let to dry. c Plasma was collected after centrifugation from the supernatant and d frozen until it was thawed for e the analysis in the routine flow of the clinical chemistry lab (cobas pro). f The dried qDBS discs were punched out and incubated with a detergent-containing buffer to extract the proteins. g The eluates of qDBS and paired plasma were analyzed using multiplexed immunoassays (Luminex). The solid lines indicate sample processing steps, and the dashed lines are comparative data analyses