Fig. 6

10mls of whole blood from ALS patients or healthy controls was collected under an IRB-approved protocol and shipped overnight at room temperature in EDTA-anticoagulated (purple top) tubes without any patient-specific confidential information. An aliquot of whole blood was taken, incubated in 1% SDS for one hour at 37 °C, prepared for SDS-PAGE, and set aside. Another aliquot of whole blood was mixed 1:1 with glucose-supplemented MEM and incubated for 72 h at 37 °C in the presence of vehicle or protein assembly modulator drugs 073 and 667, at the end of which samples were processed as previously described, and analyzed by SDS-PAGE. Black bars refer to the sample prepared for SDS-PAGE immediately, while gray bars refer to the samples incubated prior to preparation for SDS-PAGE