Fig. 5

PBMC extracts were prepared as previously, applied to drug resins and bound proteins analyzed by WB for specific proteins of interest. Bands corresponding to the correct molecular weights were quantified. Blood samples from three ALS patients were assessed over time for both p62 and RanGTPase 17 kDa fragment bound to DRAC. Timing of blood draws were 6 weeks apart for patient 78, 6 months apart for patient 50, and 9 months apart for patient 45