Table 1 Immunohistochemical protocols and scoring for IRE1, PERK, and ATF6 markers
Marker | Supplier and clone | Antigen retrieval | Primary dilution | Positive control | Interpretation | Scoring |
|---|---|---|---|---|---|---|
IRE1 | Abcam ab37073 | Microwave in pH 6.0 citrate buffer | 1:500 | Small intestine | Cytoplasmic staining | Intensity score* of cytoplasmic staining × Proportion score** of cytoplasmic staining |
PERK | Abcam ab79483 | Microwave in pH 9.0 EDTA buffer | 1:40 | Colonic carcinoma | Cytoplasmic staining and nuclear staining | (Intensity score of cytoplasmic staining × Proportion score of cytoplasmic staining)+(Intensity score of nuclear staining × Proportion score of nuclear staining) |
ATF6 | Abcam ab203119 | Microwave in pH 6.0 citrate buffer | 1:250 | Kidney | Nucleus and cytoplasmic staining | (Intensity score of cytoplasmic staining × Proportion score of cytoplasmic staining)+(Intensity score of nuclear staining × Proportion score of nuclear staining) |