Fig. 3

Comparison of different sample preparation methods: (A) Comparison of identified proteins with high significance measured in DIA mode for four different sample preparation (S-Trap with 2 and 16 h digestion time, FASP and in solution with 16 h digestion time) methods using 5 replicates of NIST plasma per experimenter (person A and B) (B) Venn diagram showing the total amount of identified proteins of the measurements mentioned in A. (C) Comparison of the mentioned sample processing procedures in regard to their identified peptides (n = 5)